2009 WSB Czech Republic – Brno 24/07/2009
| Qualifying 1 |
| Position | Rider | Manufacturer | Nation | Total Time |
| 1 | Max Biaggi | Aprilia | ITA | 1’59.982 |
| 2 | Michel Fabrizio | Ducati | ITA | 2’00.066 |
| 3 | Carlos Checa | Honda | ESP | 2’00.205 |
| 4 | Ben Spies | Yamaha | USA | 2’00.558 |
| 5 | Lorenzo Lanzi | Ducati | ITA | 2’00.581 |
| 6 | Troy Corser | BMW | AUS | 2’00.603 |
| 7 | Shinya Nakano | Aprilia | JPN | 2’00.731 |
| 8 | Ruben Xaus | BMW | ESP | 2’00.731 |
| 9 | Broc Parkes | Kawasaki | AUS | 2’00.887 |
| 10 | Yukio Kagayama | Suzuki | JPN | 2’00.958 |
| 11 | Shane Byrne | Ducati | GBR | 2’01.017 |
| 12 | John Hopkins | Honda | USA | 2’01.121 |
| 13 | Jonathan Rea | Honda | GBR | 2’01.023 |
| 14 | Jakub Smrz | Ducati | CZE | 2’01.031 |
| 15 | Noriyuki Haga | Ducati | JPN | 2’01.062 |
| 16 | Tom Sykes | Yamaha | GBR | 2’01.070 |
| 25 | David Checa | Yamaha | ESP | 2’02.785 |
